Advantages
- Rejuvenates aged muscle tissue: restoring to youthful state while enhancing muscle mass and strength.
- Reduces muscle fiber degeneration and inflammation: healthier and more effective muscle regeneration.
- Effectively reverses age-associated alterations in muscle stem cells (MuSCs), outperforming PBS treatment in aged mice models.
Summary
Sarcopenia is a condition primarily affecting the elderly, characterized by the loss of skeletal muscle mass and function. This condition is associated with impaired muscle metabolism, inflammation, and impaired muscle regeneration. There is no current effective treatment for sarcopenia on the market.
This invention introduces a novel therapeutic approach to rejuvenate aged muscle tissue using extracellular vehicles (EVs) derived from specialized muscle progenitor cells (MPCs), termed Givi-MPC, which are generated from human induced pluripotent stem cells (hiPSCs). Proteomic analysis revealed that the cargo proteins of these EVs are enriched with proteins involved in muscle regeneration, angiogenesis, and cellular repair.
This invention introduces a novel therapeutic approach to rejuvenate aged muscle tissue using extracellular vehicles (EVs) derived from specialized muscle progenitor cells (MPCs), termed Givi-MPC, which are generated from human induced pluripotent stem cells (hiPSCs). Proteomic analysis revealed that the cargo proteins of these EVs are enriched with proteins involved in muscle regeneration, angiogenesis, and cellular repair. The extracellular vesicles created from the Givi-MPC reprogram the metabolic landscape of aged muscle, enhance glutathione and tryptophan metabolism, degrade branched-chain amino acids, and increase muscle cell size and grip strength. EVs from Givi-MPC reversed the overactivation and senescent state of aged muscle stem cells, reprogramming their transcriptome towards self-renewal and differentiation upon activation. This invention presents a promising, cell-free therapeutic strategy for muscle rejuvenation.
Effect of EV on muscle regeneration by aged MuSC in tibialis anterior (TA) tissue of Mdx/scid mice after irradiation and cardiotoxin (CTX) injury. (A) Schematic outline. (B) Immunostaining for embryonic myosin heavy chain (eMyHC) and dystrophin. n=3.
Desired Partnerships
- License
- Sponsored Research
- Co-Development